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USDA/NIAID-Funded Scientists Build Chimeric Bird Flu Viruses with 100% Mortality in Mammals: Journal ‘npj Vaccines’

Gov’t finances creation of lethal avian influenza Frankenviruses in Nebraska.

A newly released npj Vaccines study confirms that U.S. government–funded researchers constructed hybrid influenza viruses in the lab and used them to trigger complete mortality in animal experiments, while framing the work under vaccine development.

The experiment, titled “Dual-Route H5N1 Vaccination Induces Systemic and Mucosal Immunity in Murine and Bovine Models,” was conducted by University of Nebraska–Lincoln scientists Joshua Wiggins, Adthakorn Madapong, and Eric A. Weaver.

You can contact the university’s Center for Virology here and the School of Biological Sciences here.

The creation of deadly chimeric pathogens was financed by the U.S. Department of Agriculture (USDA) and the National Institute of Allergy and Infectious Diseases (NIAID).

The study explicitly states:

“This research was supported by the U.S. Department of Agriculture, National Institute of Food and Agriculture, Agriculture and Food Research Initiative (Grant Nos. 2020 -06448 and 2024 -08723 to E.A.W.), and by the National Institutes of Health –NIAID (Grant No. 1R01AI147109 to E.A.W.).”

You can contact NIAID here, the NIH here, HHS here, and the USDA here to voice opposition to taxpayer-funded chimeric research on pandemic pathogens—particularly after Congress, the White House, the Department of Energy, the FBI, the CIA, and Germany’s Federal Intelligence Service (BND) all acknowledged that the deadly COVID-19 pandemic was “likely” the result of a laboratory incident involving genetically modified pathogens.

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Lab-Built ‘Chimeric’ Influenza Viruses

The researchers say they constructed the hybrid bird flu pathogens using reverse genetics.

That means the scientists assembled the viruses from scratch by inserting their genetic sequences into plasmids and introducing them into cells, which then are said to produce a fully formed infectious virus.

The paper states:

“A BSL-2 compliant reverse genetic (rg) system was used to produce” H5N1 Influenza A virus strains.”

And details how they were assembled:

“Six (PB1, PB2, PA, NP, M, and NS) IAV gene segments from the PR/8/34 H1N1 laboratory strain were cloned individually into the pHW2000 vector. Separately, the neuraminidase (N) gene and hemagglutinin (H) gene without the highly pathogenic multibasic cleavage site from each strain were synthesized and cloned into the same pHW2000 vector.”

This is a genetic recombination system:

• Internal genes from a lab strain (PR/8/34)
• Surface genes (H5N1) inserted
• Entire virus rebuilt from plasmids

That is a chimeric influenza construct—a hybrid assembled in the lab.

Engineered Pathogens Cause Lethal Disease

Even with deliberate modification of a known virulence element:

“hemagglutinin… without the highly pathogenic multibasic cleavage site”

—the viruses remained lethal.

100% Mortality in Mammals

The outcome in animals exposed to these engineered viruses is stated plainly:

“unvaccinated DPBS controls exhibited progressive weight loss… reaching 25%… requiring euthanasia.”

And:

“all control mice succumbed to infection.”

In contrast to any other framing, this is the core biological result:

• Rapid disease progression
• Severe physiological decline
• Complete mortality in unprotected animals

‘Lethal Challenge’ With Lab-Constructed Viruses

The study confirms the conditions:

“mice were challenged with lethal H5N1 reverse genetics (rg)-A/Vietnam/1203/2004(Vietnam/1203/04) or rg-A/Bovine/Ohio/B24-OSU-439/2024 (Bovine/24).”

The animals were intentionally infected with engineered lethal influenza hybrid constructs.

What the Study Actually Demonstrates

Stripped of framing, the paper shows:

• U.S. government–funded researchers
• Constructed hybrid (chimeric) influenza viruses from genetic components
• Used those constructs to induce lethal disease in mammals
• Achieved 100% mortality in controls under experimental infection

The work is presented under the justification of vaccine research.

But the underlying capability demonstrated is the intentional assembly and use of bioengineered influenza pathogens capable of killing animals.

Bottom Line

NIAID- and USDA-funded researchers say they built lab-assembled bird flu viruses by combining genetic components from multiple strains.

Then they used those engineered pathogens to infect mammals, producing rapid disease and 100% death.

Washington Joins WHO Pandemic Orchestration and Surveillance Network ‘GOARN’—Citing Bird Flu as Justification

Follows California, Illinois, Colorado, and New York City’s entry into same transnational WHO outbreak coordination system.

Governor Bob Ferguson announced this month that Washington State is now part of the World Health Organization Global Outbreak and Response Network (GOARN), an international syndicate of “public health agencies, national governments, academic centers, laboratories, and response organizations focused on rapidly detecting and responding to public health emergencies,” according to a press release from the Washington State Nurses Association (WSNA).

Washington joins California, Illinois, Colorado, and New York City by entering GOARN.

According to WSNA, Washington’s public health leaders will fall in line with the WHO’s:

• global outbreak early-warning alerts, meaning real-time surveillance tied into an international detection system
• technical collaboration and support during major public health events, meaning coordinated response across jurisdictions
• international training, exercises, and best-practice exchanges, meaning standardized response protocols
• and coordinated outbreak response support, meaning integrated deployment during declared emergencies.

Congress has already confirmed that the WHO’s response to the COVID-19 pandemic “was an abject failure” and that the WHO’s “newest effort to solve the problems exacerbated by the COVID-19 pandemic — via a “Pandemic Treaty” — may harm the United States.”

This means Washington’s decision comes despite federal findings that the WHO mismanaged the last pandemic and is advancing new agreements that could expand its influence over future responses.

You can contact Gov. Ferguson’s office here to voice your opposition to Washington’s integration into a WHO-linked outbreak surveillance and response system and demand accountability for aligning state public health infrastructure with failed global coordination mechanisms.

Washington State Governor Bob Ferguson (Governor.WA.gov)

In the governor’s press release, Washington State Secretary of Health Dennis Worsham cited avian influenza (“bird flu”) in justifying the move:

“Disease outbreaks don’t stop at state or national borders, and our ability to protect people in Washington shouldn’t either,” Washington State Secretary of Health Dennis Worsham said. “Joining GOARN ensures we maintain access to critical global outbreak intelligence and stay connected to leading public health experts, even as federal relationships change. We’re not waiting for the next threat — we’re preparing for it. From COVID-19 to rising measles cases and avian influenza, we’ve seen how quickly diseases can spread. Through GOARN, we can detect risks earlier, respond faster and better protect people in our communities — while also contributing Washington’s expertise to global response efforts.”

The development comes as bird flu is being framed internationally as an imminent threat while laboratory manipulation of the virus continues, vaccines are developed in parallel, and global, federal, and state systems are aligned to respond.

This is the same sequence of surveillance, lab work, and countermeasure rollout that preceded the COVID-19 pandemic.

Massachusetts Advances H5N1 Influenza Bird Flu Bill with Quarantine Protocols, Surveillance Grid, and Vaccination Strategies

Lawmakers move avian influenza response infrastructure into study pipeline while preserving full operational framework.

A Massachusetts bill laying out a full-scale response system for H5N1 avian influenza “bird flu” is advancing through the legislature—constructing quarantine protocols, mass surveillance systems, vaccine deployment planning, and statewide clinical trial infrastructure around a single named virus before any declared widespread outbreak.

The move comes as state, federal, and international actors are orchestrating the systems, infrastructure, and response mechanisms surrounding a future bird flu pandemic.

Massachusetts House Bill 2385 (H2385), introduced February 27, 2025 by Representative Leigh Davis (D-3rd Berkshire), does not address general pandemic preparedness.

Instead, it is specifically targeted at H5N1 bird flu, directing the state to build a coordinated response system spanning human, livestock, and wildlife populations under a single-disease framework.

On March 16, 2026, lawmakers moved the bill into a Public Health study order (H5234), advancing the proposal into a formal development phase—preserving the full framework while removing it from an immediate vote.

After sitting untouched for over a year, the bill was suddenly acted on and moved into a study process—signaling it had become important enough to preserve and develop, but not yet ready to pass in its current form.

You can contact Rep. Davis’ office here and the rest of the Massachusetts representatives here to voice your opinion on the bill.

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Emergency Bird Flu Powers Activated Up Front

The bill opens with emergency language:

“declared to be an emergency law, necessary for the immediate preservation of the public health.”

This positions the H5N1 response system for rapid activation, not gradual implementation.

Mass Bird Flu Surveillance Across Humans & Animals

The bill directs:

“developing surveillance programs to detect and track outbreaks of H5N1 in wildlife, poultry, dairy cattle and humans”

This creates a multi-species surveillance system focused specifically on bird flu, linking:

• agricultural environments
• wildlife reservoirs
• human cases

into a centralized tracking network.

Quarantine & Vaccine Plans Pre-Built for H5N1

The commission is tasked with designing:

“emergency response plans… including quarantine measures, vaccination strategies”

This establishes predefined intervention mechanisms, including:

• quarantine and isolation protocols
• movement restrictions tied to exposure
• coordinated vaccine deployment strategies

—all built specifically around bird flu response.

Statewide Bird Flu Trials Ready for Rapid Deployment

The bill calls for:

“preparation to launch statewide clinical trials that swiftly evaluate… novel therapeutic approaches”

This enables:

• rapid testing of treatments during an H5N1 event
• deployment of experimental or emerging interventions
• statewide scaling of trials

Farm Testing Network Expands Bird Flu Detection

The legislation includes:

“production of self-administered swabs… distributed to farms… allowing workers to… screen themselves”

This creates a continuous testing system tied directly to bird flu monitoring in:

• farms
• livestock environments
• worker populations

Drug Stockpiles Expand as Bird Flu Mutation Expected

The bill directs:

“shoring up of stockpiles… beyond Tamiflu… guard against viruses mutating”

This anticipates:

• mutation of H5N1
• purported need for expanded pharmaceutical reserves
• long-term treatment preparedness tied to bird flu

Private Funding Flows Into Bird Flu Response System

The commission is authorized to receive:

“funds from public and private sources such as gifts, grants and donations.”

This integrates:

• private funding sources
• external organizations
• non-government actors

into state bird flu response infrastructure.

Centralized Control System Coordinates Bird Flu Response

The bill mandates:

“coordinating efforts among government agencies… public health entities”

This establishes a centralized response model specifically for bird flu, aligning:

• public health agencies
• agriculture
• research institutions

Bill Quietly Advanced Into Study Pipeline Without Vote

The proposal remains active:

• Folded into study order H5234
• Discharged from Public Health Committee
• Transferred to the House Rules Committee

This keeps the full H5N1-focused framework moving through the legislative pipeline, positioning it for future action.

Bottom Line

H2385 is not a general preparedness bill.

It builds a targeted operational framework around avian influenza (H5N1) specifically.

The bill establishes:

• A bird flu surveillance grid across humans, livestock, and wildlife
• Predefined quarantine and vaccination protocols tied to H5N1
• A statewide clinical trial system ready for deployment
• A continuous testing pipeline in agricultural settings
• Expanded drug stockpiling anticipating mutation
• A public-private funding structure embedded in response planning
• A centralized command system for coordinated bird flu response

Lawmakers have now moved this framework into a formal study process—keeping it active and positioning it for future rollout.

China Conducts mRNA Influenza Bird Flu Vaccine Trials in Dairy Cows: Journal ‘Research’

Government preparation for large-scale mRNA vaccination of livestock.

Chinese government–funded researchers have confirmed that they tested an mRNA bird flu vaccine in lactating dairy cows, injecting milk-producing livestock with an mRNA–lipid nanoparticle formulation and then deliberately exposing the animals to live H5N1 influenza virus inside high-containment laboratories.

The peer-reviewed study published on Monday, in the journal Research, is titled “Protective Efficacy of a Hemagglutinin-Based mRNA Vaccine Against H5N1 Influenza Virus Challenge in Lactating Dairy Cows.”

The experiment signals that governments are actively preparing mRNA platforms for potential large-scale use in livestock populations, extending mRNA deployment beyond humans and into the food supply.

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mRNA Vaccine Administered to Food-Producing Animals

According to the study, researchers injected an mRNA vaccine into lactating Holstein dairy cows, meaning animals actively producing milk.

The authors write:

“Six healthy lactating dairy cows were intramuscularly immunized with 500 μg of the mRNA vaccine twice, with a 3-week interval between doses.”

The animals were sourced from an external farm:

“Lactating Holstein cows, 3 to 5 years of age, obtained from a local dairy farm, were used in the challenge experiment.”

The vaccine was an mRNA–lipid nanoparticle (mRNA–LNP) formulation encoding the hemagglutinin protein of an avian influenza virus:

“We developed a monovalent, cattle-codon-optimized mRNA–LNP vaccine encoding the HA protein.”

Deliberate Infection with H5N1 Pathogen

After receiving the mRNA injections, both vaccinated and unvaccinated cows were moved into animal biosafety level 3+ (ABSL-3+) laboratories and deliberately infected with a purportedly live H5N1 influenza virus.

The paper states:

“3 vaccinated and 3 unvaccinated lactating dairy cows were transferred into the animal biosafety level 3+ (ABSL-3+) facility for the challenge study.”

The virus was said to be administered through multiple routes, including direct injection into the mammary glands:

“All cows received a DC/24 virus challenge administered via both the intranasal and intramammary routes.”

The authors further specify:

“3 doses were directly inoculated into separate mammary quarters via the teat.”

High-Containment Facilities & Lab-Generated Viruses

All work involving the pathogen was performed inside Chinese state-authorized high-containment laboratories.

The methods section states:

“The procedures involving live HPAI viruses were performed within certified BSL-3 and ABSL-3+ laboratories at the Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences (CAAS).”

The H5N1 viruses used were not simple field samples but laboratory-generated strains.

The authors cite a prior peer-reviewed study for the virus’s creation and laboratory handling methods, rather than detailing the generation process in this paper.

“The challenge dairy cow H5N1 virus (DC/24)… was generated as previously described.”

Chinese Government Funding

The study was funded entirely by Chinese state and government research programs.

The funding disclosure reads:

“This research was funded by the National Key Research and Development Program of China… the National Natural Science Foundation of China… the Innovation Program of the CAAS… the natural science foundation of Heilongjiang Province… [and] the Central Public Interest Scientific Institution Basal Research Fund.”

All authors are affiliated with Chinese government research institutes or state-linked laboratories, including the Chinese Academy of Agricultural Sciences and China’s National High Containment Laboratory for Animal Disease Control and Prevention.

Bottom Line

The paper confirms that China is now testing mRNA vaccine platforms directly in livestock, including milk-producing animals, using live avian influenza viruses under high-containment laboratory conditions.

The study documents the use of modern mRNA technology not only in humans or laboratory animals, but in food-supply species that interface directly with agriculture, trade, and public health systems.

NIAID Funds Gain-of-Function Study Engineering Novel Influenza Viruses With New Mammalian Pathogenic and Host-Entry Functions: Journal ‘Emerging Microbes & Infections’

Despite claims the U.S. has stopped bankrolling gain-of-function experiments.

A new peer-reviewed study published this week states that federally funded researchers genetically engineered viruses that gained biological functions not present in any naturally occurring strain, including new host-entry mechanisms, cross-species antigen display, and mammalian lethality.

In multiple cases, viral surface proteins from one species and virus family were deliberately inserted into the genetic backbone of an entirely different virus, creating laboratory chimeras that bridge species and viral lineages that do not naturally mix.

The paper, “Immunogenicity and Efficacy of a Rabies-Based Vaccine against Highly Pathogenic Influenza H5N1 Virus,” appears in Emerging Microbes & Infections.

The study documents three distinct categories of functional gain:

1. transfer of influenza entry machinery into foreign viral backbones,
2. reprogramming of rabies virus to perform influenza functions, and
3. creation of new influenza chimeras that are lethal in mammals.

(Editor’s note: This article makes no claims about virology and/or terrain theory. It is reporting what NIAID-funded scientists claim to be doing with American taxdollars.)

Funding & Research Sites

The authors state:

“This study was supported by… the Center for Research on Influenza Pathogenesis and Transmission (CRIPT), one of the National Institute of Allergy and Infectious Diseases (NIAID) funded Centers of Excellence for Influenza Research and Response (CEIRR; contract # 75N93021C00014), and by NIAID contract SEM-CIVIC (contract number 75N93019C00051).”

NIAD is under the control of Director Jeffery Taubenberger, who is directing U.S. taxdollars toward influenza gain-of-function experiments while holding a patent for an influenza vaccine at the center of the Trump administrations $500 million influenza vaccine program.

This raises national security and conflict of interest concerns, as it represents the simultaneous creation of a lucrative problem and solution.

NIAD is under the authority of U.S. Health and Human Services (HHS), which is led by Robert F. Kennedy Jr.

Animal experiments were approved under:

“the Institutional Animal Care and Use Committee (IACUC) of Thomas Jefferson University (TJU).”

Influenza Host-Entry Functions Transferred Into a Different Virus

The authors state that they created a vesicular stomatitis virus whose native entry protein was replaced with influenza H5:

“VSV∆G-H5-GFP encoding either the clade 1 H5 (A/Viet Nam/1203/2004(H5N1) or the circulating clade 2.3.4.4b cow was generated as described.”

This describes a virus that now uses influenza hemagglutinin to enter host cells—a function VSV does not naturally possess.

It also represents a direct cross-species and cross-virus transfer of host-entry machinery, merging an avian influenza protein with a livestock-associated strain and a human-infecting viral backbone in a single engineered system.

Rabies Virus Reprogrammed to Display & Deliver Influenza Antigen

The study confirms that a rabies virus was engineered to express influenza H5:

“We developed a rabies virus-based H5 vaccine (RABV-H5) by insertion of a synthetic full-length codon-optimized HA ORF of the Influenza virus A/Vietnam 1203/2004(H5N1) into the BNSP333 rabies vaccine vector between the N and P genes.”

The authors further state:

“Presenting both RABV-G and the antigen of choice on the surface.”

This confirms that a neurotropic virus was genetically modified to perform a new influenza-specific function.

The lab construct combines a mammalian neurotropic virus with an avian influenza surface antigen, creating a synthetic cross-species hybrid that does not exist in nature.

Creation of Novel Influenza Viruses That Did Not Exist in Nature

The paper says that new influenza viruses were constructed by genome segment replacement:

“PR8-H5N1, a recombinant Puerto-Rico 8 influenza A virus (A/PR8) in which the HA and NA genomic segments have been replaced with the respective segments of H5N1.”

A second engineered virus is identified:

“Influenza virus A/PR8-H5N1 bovine/Ohio/439/2024 (2024).”

These viruses did not exist prior to laboratory construction.

Engineered Viruses Demonstrated Mammalian Pathogenicity

The authors report intranasal infection of mice with the engineered viruses:

“On days 104 or 150, mice were challenged by IN instillation with 0.05 ml of either 1E5 TCID50 of Influenza A/PR8-H5N1 (Viet Nam 1203 or Cow) or with 100 pfu of HPAI-H5N1 Viet Nam 1203 (2004) diluted in PBS+1% heat-inactivated FBS.”

They further confirm the dose was lethal:

“[O]n day 104 were challenged by IN instillation with a 1E5 pfu lethal dose of A/PR8-H5N1 Viet Nam 1203 virus (>100LD50).”

The paper documents viral replication in lungs:

“While unvaccinated mice had about 1E6 TCID50/ml of replicating virus in the lungs.”

And describes lung pathology:

“Severe and chronic bronchiolocentric infection with bronchiolar and peribronchiolar infiltration of lymphocytes, associated with interstitial pneumonitis and expanded alveolar wall due to edema and inflammation.”

Bottom Line

The new study makes clear that gain-of-function virus creation is allegedly still being carried out with U.S. taxpayer dollars, despite the national security and biosafety risks such work poses to the very population funding it.

Congress, the White House, the Department of Energy, the FBI, the CIA, and Germany’s Federal Intelligence Service (BND) have confirmed that the COVID-19 pandemic was likely the result of lab-engineered pathogen manipulation.

Why does the U.S. continue to fund the same experiments that are said to have caused the last pandemic?

June 19, 2025

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NIAID Director Holds Patent for Bird Flu Pandemic Vaccine—as His Agency Creates Frankenstein Bird Flu Viruses in the Lab

October 10, 2025

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Bird Flu Takes Central Role in Trump Admin’s New $500 Million ‘Next-Generation’ Pandemic Vaccine Project

May 2, 2025

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USDA–Iowa State University Lab-Engineer New Chimeric Bird Flu Viruses That Bind to Human Breast Tissue: ‘Journal of Dairy Science’

November 30, 2025

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USDA–NIH–University of Georgia–Mount Sinai Team Aerosolizes Lab-Engineered ‘Chimeric’ Influenza Frankenviruses: Journal ‘npj Vaccines’

November 28, 2025

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NIAID Funds Creation of Chimeric H5N1 Bird Flu Viruses With Modified Cleavage Sites and Enhanced Mammalian-Cell Expression: ‘npj Vaccines’

November 29, 2025

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Trump Admin Doubles Down on EO Exemptions for Gain-of-Function Experiments: Journal ‘Nature Medicine’

Jan 17

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WHO Vows ‘There Will Be Influenza Pandemics in the Future’

Jan 22

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‘Critical Loophole’ in U.S. Biosecurity Rules Allows Legal Assembly of 1918 Spanish Flu Pandemic Virus DNA: Journal ‘Nature Communications’

Jan 16

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U.S. Military Funds Intranasal Spray Self-Replicating sa-mRNA H5N1 Bird Flu Vaccine Built From Chimeric Viral Constructs: Journal ‘Nature Communications’

Jan 15

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Portugal Runs H5N1 Bird Flu Outbreak Simulation—Echoing Pre-COVID Pandemic Exercises

Jan 13

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DARPA Uses AI to Push Viral Pandemic Outbreak Modeling From Weeks to Days

Jan 9

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Bill Gates’ CEPI Revives Moderna mRNA Bird Flu Vaccine Development With $54M Investment After HHS Terminated Funding

December 19, 2025

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Saudi Arabia Bets on 2026 Bird Flu Pandemic, Ramps Up Domestic Vaccine Production Amid International H5N1 Gain-of-Function Fears

October 30, 2025

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*Article credits Jon Fleetwood